A practical rearing method for Lobesia botrana Den. & Schiff. (Lepidoptera: Tortricidae) under laboratory conditions

María E. HERRERA, Carla V. DAGATTI, Violeta C. BECERRA


Lobesia botrana Den. & Schiff. is a quarantine pest in Argentina. It was recorded in Maipú, Mendoza, in 2010. The aim of this study was the mass production of L. botrana, setting up the duration of each stage and the mortality rate. The insect rearing was developed in a chamber at 25 ± 5 °C, 30 - 50% RH, and 16: 8 (L:D) photoperiod. Under the experimental conditions, the European grapevine moths’ cycle, lasted from egg to adult death 31.27 ± 2.36 days. The incubation period was 2.82 ± 0.14 days; the larval stage lasted 13.63 ± 1.37 days. The pupal stage lasted 6.57 ± 0.22 days and the adults survived for 8.25 ± 0.64 days. The mortality egg stage was 37%, followed by the larval stage 1 with 26.30%. The other stages showed a mortality rate of less than 2.5%. Under the described conditions, the moth mass-rearing is possible. The method allows to obtain all stages. The technique used makes the rearing procedures less expensive and less time-consuming.


Artificial rearing. Quarantine pest. Vineyard.


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