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DOI: https://dx.doi.org/10.11646/phytotaxa.299.2.1

A new species of Linum (Linaceae) from Iran with a focus on description of Linum turcomanicum in view of morphological and molecular analyses

MARYAM BEHROOZIAN, JAMIL VAEZI, MOHAMMAD REZA JOHARCHI, FARSHID MEMARIANI

Abstract


The genus Linum consists of 15 species in Iran. A new species as well as a new record from Iran is described and illustrated here as L. khorassanicum and L. turcomanicum, respectively. The original description of the latter species is incomplete and ambiguous, probably due to incomplete taxon sampling. In this work, after a comprehensive taxon sampling from the entire distribution range of the species in northeast of Iran, the taxonomic description of L. turcomanicum is completed. The present study considers morphological and molecular (the nrITS region) analyses of the both species. In phylogenetic analysis based on the molecular data, we included these species and some related Linum species to examine the phylogenetic relationship of the new species and L. turcomanicum with other members of the genus. Linum turcomanicum is morphologically almost similar to L. austriacum and L. perenne, but it can be distinguished from them on the basis of several traits such as fruiting-pedicel form and length of petal. Likewise, results obtained from the molecular phylogenetic tree are consistent with those obtained from the morphological data. Linum khorassanicum is well characterized morphologically by having erect fruiting pedicels and inflorescence with few flowers. Our results suggest that the morphological data are in agreement with the molecular phylogenetic tree in which the taxonomic status of L. khorassanicum is confirmed as a new species. Based on IUCN Red List categories and criteria, L. khorassanicum and L. turcomanicum are evaluated as Endangered and Near Threatened species, respectively.

 


Keywords


biodiversity, conservation, Khorassan-Kopet Dagh, molecular phylogeny, taxonomy, Eudicots

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References


Abou El-Nasr, T.H.S. & Mahfouze, H.A. (2013) Genetic variability of golden flax (Linum usitatissimum L.) using RAPD markers. World Applied Sciences Journal 26: 851–856.

Albaladejo, R.G., Fuertes Aguilar, J., Aparicio, A. & Nieto Feliner, G. (2005) Contrasting nuclear-plastidial phylogenetic patterns in the recently diverged Iberian Phlomis crinita and P. lychnitis lineages (Lamiaceae). Taxon 54: 987–998.

https://doi.org/10.2307/25065483

Bachman, S., Moat, J., Hill, A.W., de la Torre, J. & Scott, B. (2011) Supporting Red List threat assessment with GeoCAT: geospatial conservation assessment tool. Zookeys 150: 117–126.

https://doi.org/10.3897/zookeys.150.2109

Banks, J. & Solander, D.C. (1794) Of the plants in the environs of Aleppo. In: Russell, A. (Ed.) The Natural History of Aleppo. Pater-Noster-Row, London, pp. 237–271.

Boissier, E. (1856) Diagnoses Plantarum Orientalium Novarum, ser. 2, No. 5. B. Hermann, Leipzig, 118 pp.

Braun-Blanquet, J. (1964) Pflanzensoziologie: Grundzüge der Vegetationskunde. 3. neu bearb. Aufl. , Springer-Verlag., Wien.

https://doi.org/10.1007/978-3-7091-8110-2

Cunningham, A. (1825) On the botany of the Blue Mountains. In: Field, B. (Ed.) Geographical Memories on New South Wales. John Murray, London, 504 pp.

Davis, P.H. (1967) Flora of Turkey and the East Aegean Islands. University press, Edinburgh, pp. 425–450.

Desfontaines, R.L. (1798) Flora Atlantica, Sive Historia Plantarum, quae in Atlante, Agro Tunetano et Algeriensi Crescunt. L. G. Desgranges, Paris, 444 pp.

https://doi.org/10.5962/bhl.title.323

Diederichsen, A. (2001) Comparison of genetic diversity of flax (Linum usitatissimum L.) between Canadian cultivars and a world collection. Plant Breeding 120: 360–362.

https://doi.org/10.1046/j.1439-0523.2001.00616.x

Diederichsen, A. & Raney, J.P. (2006) Seed color, seed weight and seed oil content in Linum usitatissimum accessions held by plant gene resources of Canada. Plant Breeding 125: 372–377.

https://doi.org/10.1111/j.1439-0523.2006.01231.x

Diederichsen, A., Rozhmina, T.A., Zhuchenko, A.A. & Richards, K.W. (2006) Screening for broad adaptation in 96 flax (Linum usitatissimum L.) accessions under dry and warm conditions in Canada and Russia. Plant Genetic Resources Newsletter 146: 9–16.

Diederichsen, A. & Fu, B.F. (2008) Flax genetic diversity as the raw material for future success. In: Proceedings of the International conference on flax and other best plants, July 21–23, 2008. Saskatchewan, Canada, pp. 270–280.

Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

Duminil, J., Kenfack, D., Viscosi, V., Grumiau, L. & Hardy, O.J. (2012) Testing species delimitation in sympatric species complexes: the case of an African tropical tree, Carapa spp. (Meliaceae). Molecular Phylogenetics and Evolution 62: 275–285.

https://doi.org/10.1016/j.ympev.2011.09.020

Dumortier, B.C. (1822) Reinwardtia indica. In: Dumortier, B.C. (Eds.) Commentationes Botanicae. Tournary, Ch. Casterman- Dien, pp. 19.

Farsi, M., Behroozian, M., Vaezi, J., Joharchi, M.R. & Memariani, F. (2013) The evolution of Dianthus polylepis complex (Caryophyllaceae) inferred from morphological and nuclear DNA sequence data: one or two species? Plant Systematics and Evolution 299: 1419–1431.

https://doi.org/10.1007/s00606-013-0804-z

Fu, Y.B., Peterson, G., Diederichsen, A. & Richards, K.W. (2002) RAPD analysis of genetic relationships of seven flax species in the genus Linum L. Genetic Resources and Crop Evolution 49: 253–259.

https://doi.org/10.1023/A:1015571700673

Fu, Y.B. & Allaby, R.G. (2010) Phylogenetic network of Linum species as revealed by non-coding chloroplast DNA sequences. Genetic Resources and Crop Evolution 57: 667–677.

https://doi.org/10.1007/s10722-009-9502-7

Gaudin, J. (1811) Agrostologia Helvetica. Vol. 1. Paschoud, Geneva, 361 pp.

Gill, K.S. (1987) Linseed. Publications and information Division, Indian Council of Agricultural Research, New Delhi, 386 pp.

Grisebach, A.H.R. (1843) Spicilegium Florae Rumelicae et Bithynicae Exhibens Synopsin Plantarum quas in aest. Vol. 1. Vieweg & Son, Brunsvigae, 407 pp.

Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Hassanzadeh Gorttapeh, A., Zahedmanesh, M. & Haji Hassani, M. (2007) Ecological evaluation and taxonomic identification of Linum spp. in Iran. Turkish Journal Field Crops 12: 49–55.

Hijmans, R.J., Guarino, L., Cruz, M. & Rojas, E. (2001) Computer tools for spatial analysis of plant genetic resources data: 1. DIVA-GIS. Plant Genetic Resources Newsletter 127: 15–19.

Hout, M.C., Papesh, M.H. & Goldinger, S.D. (2013) Multidimensional scaling. Wiley Interdisciplinary Reviews: Cognitive Science 4: 93–103.

https://doi.org/10.1002/wcs.1203

Huelsenbeck, J.P. & Ronquist, F. (2001) Mrbayes: Bayesian inference of phylogeny. Bioinformatics 17: 754–755.

https://doi.org/10.1093/bioinformatics/17.8.754

IUCN (2011) Guidelines for Using the IUCN Red List Categories and Criteria. Version 9.0. Prepared by the Standards and Petitions Subcommittee. Available from https://www.iucnredlist.org/ documents/RedListGuidelines.pdf (accessed 1 March 2017)

Joly, S., Starr, J.R., Lewis, W.H. & Bruneau, A. (2006) Polyploid and hybrid evolution in roses east of the Rocky Mountains. American Journal of Botany 93: 412–425.

https://doi.org/10.3732/ajb.93.3.412

Kress, W.J., Wurdack, K.J., Zimmer, E.A., Weigt, L.A. & Janzen, D.H. (2005) Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences, USA 102: 8369–8374.

https://doi.org/10.1073/pnas.0503123102

Kubitzki, K. (Ed.) (2014) The Families and Genera of Vascular Plants. Vol. XI. Flowering Plants, Eudicots: Malpighiales. Springer, Berlin, 331 pp.

Linnaeus, C. von (1753) Species Plantarum. Laurentius Salvius, Stockholm, 1200 pp.

Linnaeus, C. von (1762) Species Plantarum, ed. 2, vol. 1. Laurentius Salvius, Stockholm, 784 pp.

Mabberley, D.J. (2008) Mabberley’s Plant-book. A portable Dictionary of Plants, their Classification and Uses. Third edition. Cambridge University Press, Cambridge, 1019 pp.

McBreen, K., Lockhart, P.J., McLenachan, P.A., Scheele, S. & Robertson, A.W. (2003) The use of molecular techniques to resolve relationships among traditional weaving cultivars of Phormium. New Zealand Journal Botany 4: 301–310.

https://doi.org/10.1080/0028825X.2003.9512849

McDill, J., Repplinger, M., Simpson, B.B. & Kadereit, J.W. (2009) The phylogeny of Linum and Linaceae subfamily Linoideae, with implications for their systematics, biogeography, and evolution of heterostyly. Systematic Botany 34: 386405.

https://doi.org/10.1600/036364409788606244

Memariani, F., Zarrinpour, V. & Akhani, H. (2016a) A review of plant diversity, vegetation, and phytogeography of the Khorassan-Kopet Dagh floristic province in the Irano-Turanian region (northeastern Iran-southern Turkmenistan). Phytotaxa 249: 8–30.

https://doi.org/10.11646/phytotaxa.249.1.4

Memariani, F., Akhani, H. & Joharchi, M.R. (2016b) Endemic plants of Khorassan-Kopet Dagh floristic province in Irano-Turanian region: diversity, distribution patterns and conservation status. Phytotaxa 249: 31–117.

https://doi.org/10.11646/phytotaxa.249.1.5

Memariani, F., Joharchi, M.R. & Akhani, H. (2016c) Plant biodiversity of Ghorkhod Protected Area, NE Iran. Phytotaxa 249: 118–158.

https://doi.org/10.11646/phytotaxa.249.1.6

Mildner, R.A. & Rogers, C.M. (1978) Revision of the native South American species of Linum (Linaceae). Phytologia 39: 343–390.

Miller, P. (1768) The Gardeners Dictionary, ed. 4. Printed for the author, London, without pagination.

Müller, K. (2005) SeqState: primer design and sequence statistics for phylogenetic DNA data sets. Applied Bioinformatics 4: 65–69.

https://doi.org/10.2165/00822942-200504010-00008

Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author, Evolutionary Biology Centre, Uppsala University. Available from https://www.abc.se/~nylander/ (accessed 1 March 2017)

Ockendon, D.J. (1971) Taxonomy of the Linum perenne group in Europe. Watsonia 8: 205–235.

Özcan, T. & Zorlu, E. (2009) A contribution to taxonomy of Turkish Linum based on seed surface patterns. Biologia (Bratislava) 64: 723–730.

https://doi.org/10.2478/s11756-009-0123-6

Page, D.M. (2001) TreeView (Win32) Version 1.6.6. Available from https://taxonomy.zoology.gla.ac.uk/rod/rod.html (accessed 1 March 2017)

Planchon, J.E. (1847) Sur la Famille des Linées. The London Journal of Botany 6: 588–603.

Planchon, J.E. (1848) Sur la Famille des Linées. The London Journal of Botany 7: 165–186.

Post, G.E. (1892) Plantae Postianae, vol. 3. G. Bridel, Lausanne, 19 pp.

Pursh, F.T. (1814) Flora Americae Septentrionalis, or A Systematic Arrangement and Description of the Plants of North America, vol. 1. White, Cochrane and Co., London, 358 pp.

Rambaut, A. & Drummond, A.J. (2007) Tracer v 1.4. Available from: https://beast.bio.ed.ac.uk/tracer (accessed 11 October 2007)

Rechinger, K.H. (1974) Flora Iranica, vol. 106. Akademische Druck-u. Verlagsanstalt, Graz, 19 pp.

Rickard-Bon, S.E. & Thompson, L.U. (2003) The role of flaxseed lignans in hormone-dependent and independent cancer. In: Muir, A.D. & Westcott, N.D. (Eds.) Flax: The Genus Linum. Taylor and Francis, London, pp. 181–203.

Rogers, C.M. (1963) Yellow flowered species of Linum in eastern North America. Brittonia 15: 97–122.

https://doi.org/10.2307/2805396

Rogers, C.M. (1981) A revision of the genus Linum in southern Africa. Nordic Journal of Botany 1: 711–722.

https://doi.org/10.1111/j.1756-1051.1981.tb01157.x

Rohlf, F.J. (2000) NTSYSpc: Numerical taxonomy and multivariate analysis system, version 2.2. Applied Biostatistics Inc., New York.

Sharifnia, F. & Assadi, M. (2001) Flora of Iran. Vol. 34. Linaceae. Research Institute of Forests and Rangelands, Tehran, 48 pp. [in Persian]

Sharifnia, F. & Albouyeh, R.M. (2002) Anatomical studies in rela­tion to taxonomy of Persian Linum species. Pakistan Journal Biological Sciences 5: 1240–1245.

https://doi.org/10.3923/pjbs.2002.1240.1245

Sheidai, M., Ziaee, S., Farahani, F., Talebi, S.M., Noormohammadi, Z. & Hasheminejad Ahangarani Farahani, Y. (2014) Infra-specific genetic and morphological diversity in Linum album (Linaceae). Biologia [Bratisl] 69: 32–39.

https://doi.org/10.2478/s11756-013-0281-4

Shishkin, B.K. (Ed.) (1949) Flora of the USSR, vol. 14. Izdatelʼstvo Akademii Nauk SSSR, Moskva- Leningrad, 747 pp.

Simmons, M.P. & Ochoterena, H. (2000) Gaps as characters in sequence-based phylogenetic analyses. Systematic Biology 49: 369–381.

https://doi.org/10.1093/sysbio/49.2.369

Stegnii, V.N., Chudinova, Y.V. & Salina, E.A. (2000) RAPD analysis of flax (Linum usitatissimum L.) varieties and hybrids of various productivity. Russian Journal of Genetics 36: 1149–1152.

Steudel, E.G. von (1821) Nomenclator Botanicus, vol. 1. I. G. Cottae, Stuttgart, 900 pp.

Swofford, D.L. (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer Press, Sunderland, Mass.

Talebi, S.M., Sheidai, M., Atri, M., Sharifnia, F. & Noormohammadi, Z. (2012) Palynological study of the genus Linum in Iran (a taxonomic review). Phytologia Balcanica 18: 293–303.

Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673–4680.

https://doi.org/10.1093/nar/22.22.4673

Vaezi, J. & Brouillet, L. (2009) Phylogenetic relationships among diploid species of Symphyotrichum (Asteraceae: Astereae) based on two nuclear markers, ITS and GAPDH. Molecular Phylogenetics and Evolution 51: 540–553.

https://doi.org/10.1016/j.ympev.2009.03.003

Vaezi, J., Behroozian, M., Memariani, F. & Joharchi, M.R. (2014) Dianthus pseudocrinitus (Caryophyllaceae), a new species from northeast of Iran identified by morphological and molecular data. Phytotaxa 156: 59–73.

https://doi.org/10.11646/phytotaxa.156.2.1

Wendel, J.F. & Albert, V.A. (1992) Phylogenetics of the cotton genus (Gossypium). Character-state weighted parsimony analysis of chloroplast-DNA restriction site data and its systematic and biogeographic implications. Systematic Botany 17: 115-143.

https://doi.org/10.2307/2419069

Waldstein, F.C. & Kitaibel, P. (1805) Descriptiones et Icones Plantarum Rariorum Hungariae, vol. 2, Schmidt, Viennae, 221 pp.

White, T.J., Bruns, T., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A guide to Methods and Applications. Academic Press, San Diego, pp. 315–322.

https://doi.org/10.1016/b978-0-12-372180-8.50042-1

Winkler, H. (1931) Linaceae, tribe I. 3. Linoideae-Eulinae. In: Engler, A. and Prantl, K. (Eds.) Die natürelichen pflanzenfamilien nebst ihren Gattungen und wichtigeren Arten, insbesondere den Nutzpflanzen, 2nd ed. Vol. 19a. W. Engelmann, Leipzig, pp. 111–120.

Xu, J., Li, T.H., Justo, A. & Ge, Z.W. (2015) Two new species of Pluteus (Agaricales, Pluteaceae) from China. Phytotaxa 233: 61–68.

https://dx.doi.org/10.11646/phytotaxa.233.1.4


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