Open Access Open Access  Restricted Access Subscription or Fee Access


DOI: http://dx.doi.org/10.11646/phytotaxa.292.3.4

Hildenbrandia jigongshanensis (Hildenbrandiaceae, Rhodophyta), a new freshwater species described from Jigongshan Mountain, China

FANG-RU NAN, JIA FENG, JUN-PING LV, QI LIU, SHU-LIAN XIE

Abstract


A new freshwater red alga, Hildenbrandia jigongshanensis, is described from materials collected on the surface of rocks in flowing water from Jigongshan Mountain, China. This new species is distinguished from the other freshwater members by significantly larger cell dimensions (9.8–19.6) × (9.9–10.4) μm and filament height (364–409 μm). Phylogenetic analysis based on sequences of the rbcL gene and the construction of 18S ribosomal RNA (rRNA) secondary structure demonstrated the separation between H. jigongshanensis and other freshwater Hildenbrandia species. Specimens of H. jigongshanensis formed a clade divergent from H. rivularis group with robust support values based on rbcL gene sequences, and was in the basal position relative to the monophyletic H. rivularis, whereas the species H. angolensis was paraphyletic and distributed among both the freshwater and marine clades. Primary structure of 18S rRNA produced phylogenetic tree with low resolutions and unresolved relationship among the freshwater Hildenbrandia species due to high sequence conservation. Secondary structure based on 18S rRNA sequences distinguished the new species by absence of 3 helices compared with common structure of H. rivularis. This species description brings the total number of recognized freshwater Hildenbrandia species in China to two.


Keywords


Freshwater, Hildenbrandia, Morphology, New species, Phylogeny, Rhodophyta, Algae

Full Text:

PDF/A (1MB)

References


Agardh, J.G. (1851) Species genera et ordines algarum: seu Descriptiones succinctae specierum, generum et ordinum, quibus algarum regnum constituitur (Vol. 2). CWK Gleerup, Lund, 576 pp.

Bird, C.J. & Mclachlan, J.L. (1992) Seaweed flora of the Maritimes. 1. Rhodophyta-The red algae. Biopress, Bristol, 177 pp.

Bourrelly, P. (1985) Les AlguesD’eauDouce, Initiation À laSystématique. Tome III: les Algues Bleueset Rouges. Société Nouvelle des ÉditionsBoubée, Paris, 512 pp.

Chou, J.Y., Wen, Y.D. & Wang, W.L. (2014) Morphological and molecular data confirm new records of three freshwater red algae, Batrachospermum macrosporum, Nemalionopsis tortuosa and Caloglossa leprieurii in Taiwan. Nova Hedwigia 98: 233–246.

https://doi.org/10.1127/0029-5035/2013/0151

De Rijk, P., Wuyts, J. & De Wachter, R. (2003) RnaViz 2: an improved representation of RNA secondary structure. Bioinformatics 19: 299–300.

https://doi.org/10.1093/bioinformatics/19.2.299

Entwisle, T.J., Vis, M.L., Chiasson, W.B., Necchi Jr, O. & Sherwood, A.R. (2009) Systematics of the Batrachospermales (Rhodophyta) -A synthesis. Journal of Phycology 45: 704–715.

https://doi.org/10.1111/j.1529-8817.2009.00686.x

Felsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. Journal of Molecular Evolution 17: 368–376.

https://doi.org/10.1007/BF01734359

Freshwater, D.W., Fredericq, S., Butler, B.S., Hommersand, M.H. & Chase, M.W. (1994) A gene phylogeny of the red algae (Rhodophyta) based on plastid rbcL. Proceedings of the National Academy of Sciences o the United States of America 91: 7281–7285.

https://doi.org/10.1073/pnas.91.15.7281

Gonzalez, P. & Labarère, J. (2000) Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains. Microbiology 146: 209–221.

https://doi.org/10.1099/00221287-146-1-209

Gruber, A.R., Lorenz, R., Bernhart, S.H., Neuböck, R. & Hofacker, I.L. (2008) The vienna RNA websuite. Nucleic Acids Research 36: 70–74.

https://doi.org/10.1093/nar/gkn188

Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52: 696–704.

https://doi.org/10.1080/10635150390235520

Irvine, L.M. & Pueschel, C.M. (1994) Hildenbrandiales. In Irvine, L.M. & Chamberlain, Y.M. (Eds) Seaweeds of the British Isles, Vol. 1, Rhodophyta, part 2B, Corallinales, Hildenbrandiales. The Natural History Museum, London, pp. 235–241.

Jao, C.C. (1941) Studies on the freshwater algae of China. Sinensia 12: 245–289.

Kahn, M. (1974) On a fresh water Hildenbrandia Nardo from India. Hydrobiologia 44: 237–240.

https://doi.org/10.1007/BF00187272

Müller, K.M., Oliveira, M.C., Sheath, R.G. & Bhattacharya, D. (2001) Ribosomal DNA phylogeny of the Bangiophycidae (Rhodophyta) and the origin of secondary plastids. American Journal of Botany 88: 1390–1400.

https://doi.org/10.2307/3558445

Müller, K.M., Sheath, R.G., Vis, M.L., Crease, T.J. & Cole, K.M. (1998) Biogeography and systematics of Bangia (Bangiales, Rhodophyta) based on the Rubisco spacer, rbcL gene and 18S rRNA gene sequences and morphometric analyses. 1. North America. Phycologia 37: 195–207.

https://doi.org/10.2216/i0031-8884-37-3-195.1

Necchi Jr, O. & Zucchi, M.R. (1995) Systematics and distribution of freshwater Audouinella (Acrochaetiaceae, Rhodophyta) in Brazil. European Journal of Phycology 30: 209–218.

https://doi.org/10.1080/09670269500650991

Necchi Jr, O. (1987) Estudossobre as Rodophyta de ãguascontinentais do Brasil - 6. Ocorrência de Hildebrandia rivularis (Liebmann) J. Agardh nos Estados do Rio de Janeiro e São Paulo. Nerítica 2:147–152.

Necchi Jr, O., Sheath, R.G. & Cole, K.M. (1993) Systematics of freshwater Audouinella (Acrochaetiaceae, Rhodophyta) in North America. 1. The reddish species. Arch Hydrobiological Algological Studies Supplement 70: 11–28.

Nelson, W.A., Broom, J.E. & Farr, T.J. (2001) Four new species of Porphyra (Bangiales, Rhodophyta) from the New Zealand region described using traditional characters and 18S rDNA sequence data. Cryptogamie Algologie 22: 263–284.

https://doi.org/10.1016/S0181-1568(01)01060-1

Nichols, H.W. (1965) Culture and development of Hildenbrandia rivularis from Denmark and North America. American Journal of Botany 52: 9–15.

https://doi.org/10.2307/2439969

Posada, D. & Buckley, T.R. (2004) Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology 53: 793–808.

https://doi.org/10.1080/10635150490522304

Ragan, M.A., Bird, C.J., Rice, E.L., Gutell, R.R., Murphy, C.A. & Singh, R.K. (1994) A molecular phylogeny of the marine red algae (Rhodophyta) based on the nuclear small-subunit rRNA gene. Proceedings of the National Academy of Sciences o the United States of America 91: 7276–7280.

https://doi.org/10.1073/pnas.91.15.7276

Rintoul, T.L., Sheath, R.G. & Vis, M.L. (1999) Systematics and biogeography of the Compsopogonales (Rhodophyta) with emphasis on the freshwater families in North America. Phycologia 38: 517–527.

https://doi.org/10.2216/i0031-8884-38-6-517.1

Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.

https://doi.org/10.1093/bioinformatics/btg180

Salomaki, E.D., Kwandrans, J., Eloranta, P. & Vis, M.L. (2014) Molecular and morphological evidence for Sheathia gen. nov. (Batrachospermales, Rhodophyta) and three new species. Journal of Phycology 50: 526–542.

https://doi.org/10.1111/jpy.12179

Saunders, G.W. & Kraft, G.T. (1994) Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 1. Evidence for the Plocamiales ord. nov. Canadian Journal of Botany 72: 1250–1263.

https://doi.org/10.1139/b94-153

Saunders, G.W. (1993) Gel purification of red algal genomic DNA: an inexpensive and rapid method for the isolation of polymerase chain reaction-friendly DNA. Journal of Phycology 29: 251–254.

https://doi.org/10.1111/j.0022-3646.1993.00251.x

Sheath, R.G., Kaczmarczyk, D. & Cole, K.M. (1993) Distribution and systematics of freshwater Hildenbrandia (Rhodophyta, Hildenbrandiales) in North America. European Journal of Phycology 28: 115–121.

https://doi.org/10.1080/09670269300650191

Sherwood, A.R., Shea, T.B. & Sheath, R.G. (2002) European freshwater Hildenbrandia (Hildenbrandiales, Rhodophyta) has not been derived from multiple invasions from marine habitats. Phycologia 41: 87–95.

https://doi.org/10.2216/i0031-8884-41-1-87.1

Sherwood, A.R. & Sheath, R.G. (1999) Biogeography and systematics of Hildenbrandia (Rhodophyta, Hildenbrandiales) in North America: inferences from morphometrics and rbcL and 18S rRNA gene sequence analyses. European Journal of Phycology 34: 523–532.

https://doi.org/10.1080/09541449910001718881

Sherwood, A.R. & Sheath, R.G. (2000) Biogeography and systematics of Hildenbrandia (Rhodophyta, Hildenbrandiales) in Europe: inferences from morphometrics and rbcL and 18S rRNA gene sequence analyses. European Journal of Phycology 35: 143–152.

https://doi.org/10.1017/S0967026200002730

Sherwood, A.R. & Sheath, R.G. (2000) Microscopic analysis and seasonality of gemma production in the freshwater red alga Hildenbrandia angolensis (Hildenbrandiales, Rhodophyta). Phycological Research 48: 241–249.

https://doi.org/10.1111/j.1440-1835.2000.tb00220.x

Sherwood, A.R. & Sheath, R.G. (2003) Systematics of the Hildenbrandiales (Rhodophyta): Gene sequence and morphometric analyses of global collections. Journal of Phycology 39: 409–422.

https://doi.org/10.1046/j.1529-8817.2003.01050.x

Shi, Z.X. (2006) Flora algarum sinicarum aquae dulcis, Tomus XIII, Rhodophyta, Phaeophyta. 1st ed. Beijing Science Press, Beijing, 208 pp.

Silva, P.C., Basson, P.W. & Moe, R.L. (1996) Catalogue of the benthic marine algae of the Indian Ocean (Vol. 79). Univ of California Press, Oakland, 1259 pp.

Starmach, K. (1969) Growth of thalli and reproduction of the red alga Hildenbrandia rivularis (Liebm.) J. Ag. Acta Societatis Botanicorum Poloniae 38: 523–533.

https://doi.org/10.5586/asbp.1969.044

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731–2739.

https://doi.org/10.1093/molbev/msr121

Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876–4882.

https://doi.org/10.1093/nar/25.24.4876

Vis, M.L. & Sheath, R.G. (1996) Distribution and systematics of Batrachospermum (Batrachospermales, Rhodophyta) in North America. 9. Section Batrachospermum: description of five new species. Phycologia 35: 124–134.

https://doi.org/10.2216/i0031-8884-35-2-124.1

Vis, M.L. & Sheath, R.G. (1997) Biogeography of Batrachospermum gelatinosum (Batrachospermales, Rhodophyta) in North America based on molecular and morphological data. Journal of Phycology 33: 520–526.

https://doi.org/10.1111/j.0022-3646.1997.00520.x

Vis, M.L., Saunders, G.W., Sheath, R.G., Dunse, K. & Entwisle, T.J. (1998) Phylogeny of the Batrachospermales (Rhodophyta) inferred from rbcL and 18S ribosomal DNA gene sequences. Journal of Phycology 34: 341–350.

https://doi.org/10.1046/j.1529-8817.1998.340341.x

West, W. & West, G.S. (1897) Welwitsch’s African freshwater algae. Journal of Botany 35: 1–7.

Zeller, G. (1873) Algae collected by Mr. S. Kurz in Arracan and British Burma, determined and systematically arranged by Dr. G. Zeller. Journal of the Asiatic Society of Bengal 42: 175–193.

Zhu, H., Leliaert, F., Zhao, Z.J., Xia, S., Hu, Z.Y. & Liu, G.X. (2015) Ulvella tongshanensis (Ulvellaceae, Chlorophyta), a new freshwater species from China, and an emended morphological circumscription of the genus Ulvella. Fottea 15: 95–104.

https://doi.org/10.5507/fot.2015.008


Refbacks

  • There are currently no refbacks.


ISSN 1179-3155 (print); ISSN 1179-3163 (online)

Published by Magnolia Press, Auckland, New Zealand