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DOI: https://dx.doi.org/10.11646/phytotaxa.166.4.3

Occurrence of true branches in Rhizoclonium (Cladophorales, Ulvophyceae) and the reinstatement of Rhizoclonium pachydermum Kjellman

ZHI-JUAN ZHAO, HUAN ZHU, ZHENG-YU HU, GUO-XIANG LIU

Abstract


The phylogenetic position of the freshwater green alga Rhizoclonium pachydermum (Ulvophyceae: Cladophorales) was investigated using nuclear 18S rRNA gene and internal transcribed spacer 2 (ITS2) sequences. This alga has been referred to as Cladophora pachyderma. Based on its morphology, it was formerly classified in the section Affines in the genus Cladophora. However, this classification was not supported by the current phylogenetic analyses, where Rhizoclonium pachydermum formed a well-supported clade with other Rhizoclonium species. We consider that Rhizoclonium possesses real branches and the most important criteria that characterize the genus are: long unbranched filaments only with rhizoid branches, or only branched at the basal region of the thallus; and cylindrical cells with few or limited numbers of nuclei.


Keywords


18S rDNA, Cladophorales, ITS2, phylogeny, Rhizoclonium pachydermum, taxonomy

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References


Agardh, C.A. (1827) Aufzählung einiger in den östreichischen Ländern gefundenen neuen Gattungen und Arten von Algen, nebst ihrer Diagnostik und beigefügten Bemerkungen. Flora 10: 625–640.

Balakrishnan, M.S. (1961) A cytotaxonomical investigation of Indian members of the Cladophoraceae. In: Mahabale, T.S. (ed.) Professor S.P. Agharkar Commemoration Volume. University of Poona, Poona, pp. 34–40.

Boedeker, C. (2012) Phylogenetic, taxonomic and biogeographical studies in the Pithophoraceae (Cladophorales, Chlorophyta). Journal of Phycology 48: 808–825.

https://dx.doi.org/ 10.1111/j.1529-8817.2012.01145.x

Borzi, A. (1883) Studl algologici. Fasc. I. Messina, 112 pp.

Brand, F. (1909) Zur Morphologie und Biologie des Grenzgebietes zwischen den Algengattungen Rhizoclonium und Cladophora. Hedwigia 48: 45–73.

Brand, F. (1913) Ueber Cladophora humida n. sp., Rhizoclonium lapponicum n. sp., und deren bostrychoide verzweigung. Hedwigia 57: 179–183.

Chapman, V.J. (1956) The marine algae of New Zealand, part I, Myxophyceae and Chlorophyceae. Botanical Journal of the Linnean Society 55: 333–501.
https://dx.doi.org/10.1111/j.1095-8339.1956.tb00019.x

Christensen, T. (1991) On some Cladophoraceae (Chlorophyta) with long unbranched filaments. Archiv fur Protistenkunde 139: 9–19.
https://dx.doi.org/10.1016/s0003-9365(11)80003-5

Delile, A.R. (1813). Florae Aegyptiacae illustratio. In France (Commission d’Egypte), Description de l’Egypte ou recueil des observations et des recherches qui ont e´te´ faites en Egypte pendant l’expe´dition de l’arme´e francaise (1798 – 1801), Histoire naturelle 2, pp. 49–82, 145–320 + atlas, pl. 1–62.

Doyle, J.J. & Dickson, E.E. (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36: 715–722.
https://dx.doi.org/10.2307/1221122

Gardavsky, A. (1993) Rhizoclonium fractiflexum sp. nova, a new member of Cladophorales (Chlorophyta) described from freshwater aquaria. Archiv fur Protistenkunde 143: 125–136.
https://dx.doi.org/10.1016/S0003-9365(11)80281-2

Gay, F. (1891) Le genre Rhizoclonium. Journal of Botany (Paris) 5: 53–58.

Hall, T.A. (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Harvey, W.H. (1849) Phycologia Britannica. London, pl. 121–240.

Hayakawa, Y., Ogawa, T., Yoshikawa, S., Ohki, K. & Kamiya, M. (2012) Genetic and ecophysiological diversity of Cladophora (Cladophorales, Ulvophyceae) in various salinity regimes. Phycological Research 60: 86–97.
https://dx.doi.org/ 10.1111/j.1440-1835.2012.00641.x

Huelsenbeck, J.P. & Crandall, K.A. (1997) Phylogeny estimation and hypothesis testing using maximum likelihood. Annual Review of Ecology, Evolution and Systematics 28: 437–466. https://dx.doi.org/10.1146/annurev.ecolsys.28.1.437

Huelsenbeck, J.P. & Ronquist F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754–755.
https://dx.doi.org/10.1093/bioinformatics/17.8.754

Ichihara,K., Shimada, S. & Miyaji K. (2013) Systematics of Rhizoclonium–like algae (Cladophorales, Chlorophyta) from Japanese brackish waters, based on molecular phylogenetic and morphological analyses. Phycologia 52: 398–410. https://dx.doi.org/10.2216/12-102.1

Jao, C.C. (1947) Studies on the freshwater algae of China XVII: Ulotrichales, Siphonocladiales, and Siphonales from Kwangsi. Botanical Bulletin of Academia Sinica 1: 257–269.

Kann, E. (1947) Zur Ökologie der Litoralalgen in ostholsteinischen Waldseen. Archiv für Hydrobiologie 41: 14–42.

Kjellmann, F.R. (1877) Ueber die Algenvegetation des Murmanschen Meeres an der Westkste von Nowaja Semlja und Wajgatsch. Nova Acta Regiae Societatis Scientiarum Upsaliensis Volumen Extraordinem 12: 1–85.
https://dx.doi.org/10.5962/bhl.title.64252

Kützing, F.T. (1843) Phycologia generalis. Leipzig, 458 pp.

Kützing, F.T. (1845) Phycologia germanica. Nordhausen, 240 pp.

Kützing, F.T. (1849) Species Algarum. Brockhaus, Leipzig, 922 pp.

Leliaert, F., & Boedeker, C. (2007) Cladophorales. In: Brodie, J., Maggs, C.A. & John, D.M. (eds.) Green seaweeds of Britain and Ireland. Natural History Museum Publications, London, pp. 131–183.

Leliaert, F., De Clerck, O., Verbruggen, H., Boedeker, C. & Coppejans, E. (2007) Molecular phylogeny of the Siphonocladales (Chlorophyta: Cladophorophyceae). Molecular Phylogenetics and Evolution 44: 1237–1256.
https://dx.doi.org/10.1016/j.ympev.2007.04.016

Liu, G.X. & Hu, Z.Y. (1999) Three new records of freshwater Cladophorales from China. Acta Hydrobiologica Sinica 23: 191–194.

Luo, W., Pflugmacher, S., Pröschold, T., Walz, N. & Krienitz, L. (2006) Genotype versus phenotype variability in Chlorella and Micractinium (Chlorophyta, Trebouxiophyceae). Protist 157: 315–333.
https://dx.doi.org/10.1016/j.protis.2006.05.006

Mathews, D.H.; Sabina, J., Zuker, M. & Turner, D.H. (1999) Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. Journal of Molecular Biology 288: 911–940.
https://dx.doi.org/10.1006/jmbi.1999.2700

Nienhuis P.H. (1975) Biosystematics and ecology of Rhizoclonium riparium (Roth) Harvey (Chlorophyceae: Cladophorales) in the estuarine area of the rivers Rhine, Meuse, and Scheldt. Bronder Offset B.Y., Rotterdam, 240 pp.

Parodi, E.R. & Caceres, E.J. (1993) Life history of freshwater populations of Rhizoclonium hieroglyphicum (Cladophorales, Chlorophyta). European Journal of Phycology 28: 69–74.
https://dx.doi.org/10.1080/09670269300650111

Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817–818.
https://dx.doi.org/ 10.1093/bioinformatics/14.9.817

Prescott, G.W. (1982) Algae of the western Great Lakes area. Otto Koeltz Science Publishers, Koenigstein, 998 pp.

Roth, A.G. (1806) Catalecta botanica quibus plantae novae et minus cognitae describuntur atque illustrantur. Fasciculus tertius cum tabulis aenaeis XII. Leipzig, 350 pp.

Scagel R.F. (1966) Marine algae of British Columbia and Northern Washington, Part I: Chlorophyceae (green algae). National Museum of Canada Bulletin 207, Ottawa, vii + 257 pp.

Schmidle, W. (1897) Beiträge zur Algenflora des Schwarzwaldes und Oberrheins VI. Hedwigia 36: 1–25.

Setchell, W.A. & Gardner, N.L. (1920) The marine alage of the Pacific coast of North America. Part. 2, Chlorophyceae. University of California of publications in Botany 8: 139–374.

Swindell, S.R. & Plasterer, T.N. (1997) SEQMAN: Contig assembly. Methods in Molecular Biology 70: 75–89.
https://dx.doi.org/10.1385/0-89603-358-9:75

Swofford, D.L. (2003) PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0b 10. Sinauer Associates, Sunderland.

van den Hoek, C. & Chihara, M. (2000) A taxonomic revision of the marine species of Cladophora (Chlorophyta) along the coasts of Japan and the Russion Far–East. National Science Museum, Tokyo, 242 pp.

van den Hoek, C. (1963) Revision of the European species of Cladophora. Brill, Leiden. 294 pp.

van den Hoek, C. (1982) A taxonomic revision of the American species of Cladophora (Chlorophyceae) in the north Atlantic ocean and their geographic distribution. North-Holland Publishing, Amsterdam, 329 pp.

van den Hoek, C. (1984) The systematics of the Cladophorales. In: Irvine, D.E.G. & John, D.M. (eds.) Systematics of the green algae. Academic Press, London and Orlando, pp. 157–178.

Weber, F. & Mohr, D.M.H. (1804) Naturhistorische Reise durch einen Theil Schwedens. Göttingen, pp. [i]-xii, [13] -207 [-208].

West, W. & West, G.S. (1897) Welwitsch’s African freshwater algae. Journal of Botany, British and Foreign 35: 1–7, 33–42, 77–89, 113–122, 172–183, 235–243, 264–272, 297–304, pls. 365–369.

Wittrock, V.B., Nordstedt, C.F.O. & Lagerheim, G. (1896) Algae aquae dulcis exsiccatae praecipue scandinavicae quas adjectis algis marinis chlorophyllaceis et phycochromaceis 26–29: 1201–1400.

Womersley, H.B.S. (1984) The marine benthic flora of southern Australia Part I. Government Printer, Adelaide, South Australia, 329 pp.


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